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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: DNA Repair (Amst). 2019 Jan 16;75:1–17. doi: 10.1016/j.dnarep.2019.01.002

Fig. 3. Testing the contribution of ribo-thymine from stable RNA.

Fig. 3.

A. A scheme of stable RNAs (tRNA has one rT, 23S rRNA has two) and how many thymine residues they can yield per average rapidly-growing cell. B. Time course of TLD after 15’ @ 54°C heat shock. The strain is KKW58. In this case, the experiment-specific thyA TLD curve was used. C. Stability of tRNA and rRNA during thymine starvation. The strain is KKW58. Stability is expressed as the ratio of the corresponding RNA species in the cells incubated without dT to cells from the same culture incubated in the presence of dT. The actual measurements, normalized to time 0, are shown in Figures S3B and S3C. D. Recruitment of rT by degrading stable RNA kills two birds with one stone: it yields dT to support stalling replication forks and at the same time it inhibits translation, blocking new initiations and slowing down general metabolism. Both changes contribute to metabolism rebalancing. E. Time course of TLD in the thyA trmA mutant (RA9). F. Time course of TLD in the thyA rumA rumB mutant (RA13). G. Time course of TLD in the trmA thyA and trmA rumB thyA mutants. The strains are: thyA, KKW58; trmA thyA, RA9 (from “E”); trmA rumB thyA, RA14. H. Evolution of the chromosomal DNA absolute amount in the thyA and trmA thyA mutants (strains like in “E”) during T-starvation.