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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: DNA Repair (Amst). 2019 Jan 16;75:1–17. doi: 10.1016/j.dnarep.2019.01.002

Fig. 4. The role of deoxy-uridine in TLD.

Fig. 4.

A. A scheme of DNA-dU misincorporation and its consequence in thyA, ung thyA, dut thyA and dut ung thyA mutants undergoing thymine starvation. Green fill, growth with dT. Light pink fill, attempted replication without dT using little dU (Dut+) with active DNA-uracil removal (Ung+). Yellow fill, replication without dT using little dU (Dut+), with no DNA-uracil removal (ung). Pink fill, attempted replication without dT but with plenty of dU (dut) with active DNA-uracil removal (Ung+). Light green fill, normal replication without dT but with plenty of dU (dut) and without DNA-uracil excision (ung). B. Time course of TLD in ABS-endo mutants. Strains are: thyA, KKW58; xthA thyA, RA22; nfo thyA, RA23; xthA nfo thyA, RA24. C. Time course of TLD in the dut thyA and ung thyA mutants. The +dT/-dT medium switch in this case was by centrifugation. Strains are: thyA, KKW58; dut thyA, RA16; ung thyA, KJK78; dut ung thyA, RA18. D. Evolution of the chromosomal DNA amount in the thyA (KKW58) and dut ung thyA (RA18) mutants during thymine starvation. E. Evolution of the replication origin copy number in the thyA and dut ung thyA mutants (strains like in “D”) during thymine starvation. F. Evolution of the chromosomal terminus copy number in the thyA and dut ung thyA mutants (strains like in “D”) during thymine starvation. G. TLD kinetics of the recF dut ung thyA mutant. The strains are thyA,KKW58; thyA recF, RA31; thyA dut ung, RA18; thyA dut ung recF, RA32.