Skip to main content
. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: DNA Repair (Amst). 2019 Jan 16;75:1–17. doi: 10.1016/j.dnarep.2019.01.002

Fig. 7. Accumulation of ss-gaps during T-starvation.

Fig. 7.

A. Evolution of the chromosomal DNA amount in the thyA (KKW58) versus recF thyA (RA31) mutants during T-starvation. B. An example of plug-blot procedure to quantify ssDNA accumulation during T-starvation. After electric transfer to positively-charged nylon membrane, the genomic DNA was hybridized to the total genomic probe. C. Accumulation of ssDNA in percentage to the total DNA signal in the WT cell (AB1157) cultures grown in the presence of the indicated concentrations of AZT. D. The level of ssDNA in thyA mutant (KKW58) cultures grown in the presence (+dT) or absence (–dT) of thymidine. E. The level of ssDNA in the thyA recF (RA31) mutant. F. The level of ssDNA in the thyA dut ung (RA18) mutant.