Figure 1. Gα_i2 signaling regulates inflammasome activation in BMDMs.

BMDMs were generated from WT and A) Gα_i2 KO (Gnai2^−/−), B) Ric-8 KO (Ric8^−/−), C) G184S KI (Gnai2^G184S/G184S), or D) RGS10 KO (Rgs10^−/−) mice. BMDM inflammasomes were activated as indicated in the methods section, and supernatant IL-1β assayed by ELISA. The graphs shown are representative of 5 separate experiments with three replicates. P values were determined using Student’s t-test and p < 0.05 considered statistically significant (* p <0.05; ** p<0.005, *** p<0.001).