Figure 2. Gα_i3 deficiency does not affect inflammasome activity in macrophages.

BMDMs were generated from WT and A) Gα_i3 KO (Gnai3^−/−) mice, B) RGS1 KO (Rgs1^−/−), C) RGS19 KO (Rgs19 ^−/−), or D) AGS3 KO (Gpsm1^−/−) mice. BMDM inflammasomes were activated as indicated in the methods section, and the supernatant IL-1β assayed by ELISA. The graphs shown are representative of 5 separate experiments with three replicates. P values were determined using Student’s t-test and p < 0.05 considered statistically significant (* p <0.05; ** p<0.005, *** p<0.001).