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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Immunol. 2019 Jan 25;202(5):1510–1520. doi: 10.4049/jimmunol.1801145

Figure 5. Long-term, but not short term, inhibition of Gαi2 signaling recapitulates the phenotypes seen in genetically altered BMDMs.

Figure 5.

A) BMDMs from WT mice treated at only day 7 with PTX (200 ng/ml) overnight before inflammasome activation. BMDM culture supernatants were collected and IL-1β assayed by ELISA. The bar graphs shown are representative of five separate experiments with three replicates. B) Expression of M1 genes in PTX treated BMDMs (50 ng/ml for days 0–2; 100 ng/ml for days 3–7) after treatment with LPS (1 μg/ml) for 4 hours. C) Expression of M2 genes in PTX treated BMDMs (50 ng/ml for days 0–2; 100 ng/ml for days 3–7) after treatment with IL-4 (20 ng/ml) for 48 hours. RT-PCR experiments were conducted at least 3 times in triplicate. Data is presented as fold change relative to the WT control. P values were determined using Student’s t-test and p < 0.05 was considered statistically significant (* p <0.05; ** p<0.005, *** p<0.001).