FIGURE 5.

Akt1^–/– and Akt1^–/– Tregs display differential suppressive activity in vitro and in vivo. (A) CD4⁺CD25⁺ Tregs of WT, Akt1^–/– and Akt1^–/– mice were cultured with CFSE-labeled naïve CD4⁺CD25⁻ Teffs in the presence of plate-bound anti-CD3 and anti-CD28 for 96 h. The proliferation of Teffs was determined by flow cytometry. (B) Naïve CD4⁺CD25⁻ T cells from WT mice together with CD4⁺CD25⁺ T cells from WT, Akt1^–/–, or Akt1^–/– mice were adoptively transferred into Tcrb^–/– mice (n = 5 per group) which were then immunized with MOG_35–55 in CFA. The development of EAE was monitored. * p < 0.05; Mann-Whitney U test. (C) The CD4⁺IFN-γ⁺, CD4⁺IL-17⁺ T cells, and CD4⁺CD25⁺Foxp3⁺ T cells within the draining lymph node cells of the recipients were determined. * p< 0.05; Student t test. The data shown are one representative of three independent experiments.