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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Immunol. 2019 Jan 25;202(5):1635–1643. doi: 10.4049/jimmunol.1800765

Figure 1. CD163 mRNA is expressed in human blood monocytes and THP-1 monocytic cells and increases in response to IL-10 treatment.

Figure 1.

Purified CD14+ human monocytes or THP-1 cells were left unstimulated or stimulated with 50 ng/ml IL-10 for 24 hours. Cells were stained for CD14 expression and then processed for CD163 mRNA detection as described. (A) Density plots indicate percentages of CD163 mRNA signal from gated live singlet CD14+ monocytes (top) or THP-1 cells (bottom) acquired using a LSR Fortessa. MFI values are 332, 665, and 859 for THP-1 unstained, unstimulated, and IL-10 stimulated populations, respectively. Images are representative of three independent experiments. (B) Images are representative of singlet THP-1 cells acquired using an ImageStreamX with median intensity of CD163 gated signal indicated below. Images are representative of two independent experiments.