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. 2019 Feb 14;10:105. doi: 10.3389/fgene.2019.00105

FIGURE 1.

FIGURE 1

Definition of 5′ and 3′ sRNAs. (A) 5′ and 3′ sRNAs (in red color) are defined as sRNA-seq reads with lengths of 15∼50 bp, which are precisely aligned to the 5′ and 3′ ends of mature RNAs, respectively. The lengths of them vary progressively with 1-bp differences. This figure shows 5′ and 3′ sRNAs from a typical tRNA (in blue color). As for longer RNAs (e.g., snRNAs or rRNAs), there could be abundant sRNAs in the body. (B) 5-end format is defined to easily identify 5′ ends of mature RNAs using sRNA-seq data. (C) Human rRNA genes (RefSeq: NR_046235.1) were annotated using alignment results in the 5-end format. Among positions 7923, 7924, and 7925 with ratio1s (the 5th column) above 70%, the position 7925 with the highest ratio1 was determined as the 5′ end of 28S rRNA.