FIGURE 3.

Corrected annotations of mitochondrial tRNAs. tRNA^Tyr/tRNA^Cys/tRNA^Asn/tRNA^Ala is encoded by L-strand of human mitochondrial genome. tRNA^Ala is not showed, as it does not need correction. ncMT1 was first discovered in this study. (A) Mitochondrial tRNAs are annotated between two cleavage sites using the sRNA-seq based method, while they are annotated between two trimming sites and several nucleic acids in red color are missed using other existing methods. (B) The acceptor stem of a tRNA precursor is trimmed to contain a 1-bp overhang at the 3′ end. CCAs are post-transcriptionally added to the 3′ ends of tRNAs, one nucleotide at a time. A polyA tail is post-transcriptionally added to the 3′ end of ncMT1.