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. 2019 Feb 14;10:244. doi: 10.3389/fmicb.2019.00244

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Copyright © 2019 Fu, Qin, Zong, Liu, Kang, Zhong and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Verification of the cagRS deletion strain ΔcagRS. (A) Schematic diagram of the construction of the cagRS mutant and complemented strains. F and R represent forward and reverse primers, respectively. (B) PCR verification of the ΔcagRS mutant. M: DNA marker. The F/R primers are located at the flanking regions of the TCS. (C) Schematic diagram of CA biosynthesis-related gene clusters in F613-1. The red and yellow bidirectional arrows indicate homologous genes. The CA biosynthetic gene cluster (blue arrows) includes five operons: ceaS2-bls2, pah2-cas2, cyp450-fd, orf12-orf13 and gcaS-orf16-oppA2.