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. 2019 Feb 14;10:244. doi: 10.3389/fmicb.2019.00244

FIGURE 5.

FIGURE 5

CagRS regulates the biosynthesis of arginine-the direct precursor of CA. (A) Schematic diagram of the arginine biosynthetic gene cluster. P1–P5 are different promoter regions of the arginine gene cluster: P1, 211 bp of the argH upstream region; P2, 299 bp of the argG upstream region; P3, 154 bp of the argB-D-R upstream region; P4, 271 bp of the argJ upstream region; P5, 160 bp of the argC upstream region. (B) The binding of CagR to the promoter regions of argC and argG. EMSAs of argG, argB, argC, argJ, and argH with purified His-tagged CagR. The promoter fragments were labeled with biotin-11-UTP using the Biotin 3′ End DNA Labeling kit. The above probes were incubated either with no protein (–) or 3.0 μg CagR (+). The appropriate amount of polydI/dC (1.0 mg) was used as competitor. (C) Comparison of argG and argC promoter sequences with the conserved CGCNGCCG motif for CagR binding.