FIGURE 6.
The binding of CagR to the promoter region of CA cluster genes. (A) The upstream promoter region of CA cluster genes are, respectively: 181 bp of the claR upstream region; 214 bp of the ceaS1 upstream region; 169 bp of the oat2 upstream region; 171 bp of the oat1 upstream region; 234 bp of the ceaS2-bls2 upstream region; 196 bp of the pah2-cas2 upstream region; 158 bp of the oppA1 upstream region; 191 bp of the car upstream region; 157 bp of the cyp450-fd upstream region; 246 bp of the orf12-orf13 upstream region; 137 bp of the orf14 upstream region; 171 bp of the gcaS-orf16-oppA2 upstream region; 213 bp of the pah1 upstream region; 183 bp of the cas1 upstream region. The probes were incubated either with no protein (–) or 3.0 μg CagR (+). The appropriate amount of polydI/dC (1.0 mg) was used as competitor. (B) Comparison of claR, oat2, oat1 and ceaS1 promoter sequences with the conserved motif for CagR binding.