Figure 5.

DTLL promotes inhibition of proliferation, cycle arrest and apoptosis of pancreatic cancer cells. A, The inhibition rate of MIA‐paca‐2 cell growth was detected by MTS assay. Cells were treated with different concentrations of tested agents including DTLL, LDM, and gemcitabine at indicated concentrations for 48 h at 37°C. B, The inhibition rate of MIA‐paca‐2 cell growth was detected using a clonogenic assay with the same treatments as (A) except that the medium was replaced with drug‐free medium after 24 h. C, Flow cytometry analysis was performed to measure apoptosis of MIA‐paca‐2 cells induced by DTLL/or LDM at 0.1 and 1 nmol/L for 24 h. D, MIA‐paca‐2 cells were exposed to DTLL and LDM at different concentrations (0.01 and 0.1 nmol/L), and cell cycle distribution was determined by flow cytometry after PI staining. The SD values for three repeated experiments were calculated with GraphPad Prism 6.0 software