Fig. 5.

Trabecular bone mass is increased in PRX-HIF2dPA^f/+ mutant mice. a Micro-CT analysis of distal femur metaphyses isolated from 6- and 12-week-old male and female HIF2dPA^f/+ and PRX-HIF2dPA^f/+ mice. Representative cross-section images are shown on the left; quantification of trabecular bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th.), trabecular number (Tb.N.), and trabecular separation (Tb.Sp.) is provided on the right. Note the virtual absence of cortical bone in the mutant metaphysis. b Histomorphometric analysis of distal femur metaphyses isolated from 6- and 12-week-old male and female HIF2dPA^f/+ and PRX-HIF2dPA^f/+ mice. At the top, representative paraffin sections stained with H&E are shown. Note the unique reticular pattern of the trabeculae and the presence of dilated blood vessels in mutant bones. The latter finding is consistent with the upregulation of Vegfa and inducible nitric oxide synthase (iNOS) detected in mutant cells (Fig. 1 and Fig. 9b). Quantification of the same parameters measured in (a) is provided at the bottom. M = male; F = female. *P < 0.05 referred to the comparison of HIF2dPA^f/f and PRX-HIF2dPA^f/+ specimens matched for age and gender. Scale bars = 100 μm