Fig. 7.

Loss of HIF2 in osteoblast precursors increases both trabecular and cortical bone mass. a Micro-CT analysis of trabecular bone in distal metaphysis and cortical bone in mid-diaphysis of femurs isolated from 12-week-old male HIF2^f/f, PRX-HIF2^f/+, and PRX-HIF2^f/f mice. At the top, representative images of trabecular (on the left) and cortical bone (on the right) are shown. At the bottom, quantification of BV/TV, Tb.N., Tb.Th., Tb.Sp. CA/TA, and Ct.Th. is provided. b Static histomorphometry analysis of trabecular bone of distal femurs isolated from 12-week-old male HIF2^f/f, PRX-HIF2^f/+, and PRX-HIF2^f/f mice. At the top, H&E staining of representative histological sections is shown. At the bottom, quantification of BV/TV, Tb.N., Tb.Th., Tb.Sp., and Ob.N./BS is provided. c Dynamic histomorphometric analysis of distal femurs isolated from 12-week-old male HIF2^f/f, PRX-HIF2^f/+, and PRX-HIF2^f/f mice. On the left, representative double-calcein labeling images are shown; on the right, quantification of MAR and BFR/BS is provided. Notably, a modest augmentation of some micro-CT and static histomophometric parameters was also noted in PRX-HIF2^f/+ specimens, although in most cases, it did not reach statistical significance. *P < 0.05 referred to the comparison of HIF2^f/f and PRX-HIF2^f/f mice