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. 2019 Feb 21;7:7. doi: 10.1038/s41413-019-0045-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — Sox9 is a downstream target of HIF2 in BMSCs. a mRNA microarray analysis of genes regulated by the HIF2dPA mutant protein in BMSCs. Hierarchical representation of gene cluster distribution depending on gene expression levels. b Selected number of upregulated (red) and downregulated (blue) genes. c qRT-PCR of total RNA isolated from HIF2adPA^f/f BMSCs and transduced with Ad-LacZ or Ad-Cre. Sox9 mRNA is shown. Data were normalized to expression of TBP mRNA. d Bioinformatics analysis of the HRE motif in the Sox9 promoter. Consensus sequence (5′-[A/G]CGTG-3′) of HRE and JASPAR predicted HREs within –3 500/ + 100-bp region of the mouse Sox9 promoter are shown. e 2-LoxP qPCR of genomic DNA isolated from Sox9^f/f BMSCs and transduced with Ad-LacZ or Ad-Cre. Data were normalized to Vhl as an internal control for genomic DNA input. f qRT-PCR of total RNA isolated from Sox9^f/f BMSCs and transduced with Ad-LacZ or Ad-Cre. Sox9 and Sp7 mRNAs are shown. Data were normalized to expression of TBP mRNA. *P < 0.05