Table 2.

Primers used for qRT-PCR of total RNA

Primers	Sequence	Amplicon/bp
Tbp	Fwd 5′-AGAACAATCCAGACTAGCAGCA-3′	120
	Rev 5′-GGGAACTTCACATCACAGCTC-3′
HIF2	Fwd 5′-CTGAGGAAGGAGAAATCCCGT-3′	161
	Rev 5′-TGTGTCCGAAGGAAGCTGATG-3′
Runx2	Fwd 5′-TGTTCTCTGATCGCCTCAGTG-3′	146
	Rev 5′-CCTGGGATCTGTAATCTGACTCT-3′
Col1	Fwd 5′-TGTGTGCGATGACGTGCAAT-3′	133
	Rev 5′-GGGTCCCTCGACTCCTACA-3′
Sp7	Fwd 5′-GGAAAGGAGGCACAAAGAAGC-3′	218
	Rev 5′-CCCCTTAGGCACTAGGAGC-3′
Alpl	Fwd 5′-CGGATCCTGACCAAAAACC-3′	74
	Rev 5′-TCATGATGTCCGTGGTCAAT-3′
Ibsp	Fwd 5′-GAAAATGGAGACGGCGATAG-3′	73
	Rev 5′-CATTGTTTTCCTCTTCGTTTGA-3′
Spp1	Fwd 5′-AGCAAGAAACTCTTCCAAGCAA-3′	134
	Rev 5′-GTGAGATTCGTCAGATTCATCCG-3′
Ocn	Fwd 5′-AGACTCCGGCGCTACCTT-3′	85
	Rev 5′-AAGCAGGGTCAAGCTCACAT-3′
Opg	Fwd 5′-GTTTCCCGAGGACCACAAT-3′	71
	Rev 5′-CCATTCAATGATGTCCAGGAG-3′
Rankl	Fwd 5′-TGAAGACACACTACCTGACTCCTG-3′	84
	Rev 5′-CCCACAATGTGTTGCAGTTC-3′
Vegfa	Fwd 5′-CTTGTTCAGAGCGGAGAAAGC-3′	125
	Rev 5′-ACATCTGCAAGTACGTTCGTT-3′
Col2a1	Fwd 5′-ACTTGCCAAGACCTGAAACTCTG-3′	97
	Rev 5′-AAACTTTCATGGCGTCCAAGG-3′
Acan	Fwd 5′-CCTGCTACTTCATCGACCCC-3′	150
	Rev 5′-AGATGCTGTTGACTCGAACCT-3′
Sox9	Fwd 5′-AGTACCCGCATCTGCACAAC-3′	145
	Rev 5ʹ-TACTTGTAATCGGGGTGGTCT-3′
Glut1	Fwd 5ʹ-CAGTTCGGCTATAACACTGGTG-3′	156
	Rev 5′-GCCCCCGACAGAGAAGATG-3′
Hif1a	Fwd 5′-TCTCGGCGAAGCAAAGAGTC-3′	214
	Rev 5′-AGCCATCTAGGGCTTTCAGATAA-3′