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. 2019 Feb 20;9:2339. doi: 10.1038/s41598-019-38800-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCA plots of the intestinal microbiome. The four panels show the bacterial community at the end of the second feeding period, represented by pseudo-variables generated during Principal Component Analysis in relation to the 1^st and 2^nd Feeding Diets. Each object represents one individual fish. Additionally, the panels (a–d) present the relative abundances of Vibrionales, Lactobacillales, Clostridiales and Bifidobacteriales, respectively, for each fish. The shape of objects represents the 1^st Feeding Diet; the colours indicate the 2^nd Feeding Diet and the size of each object relates to the relative abundance of the bacterial order that is indicated in the legend of each panel.