Fig. 4.

Diclofenac promotes cardiac reprogramming via inhibition of the PGE2/EP4 pathway. a, b FACS analyses for αMHC-GFP and cTnT expression. Postnatal αMHC-GFP TTFs were transduced with GHMT and cultured with the indicated prostanoids for 1 week. Quantitative data are shown in b; n = 3 biologically independent experiments. c–e FACS analyses for αMHC-GFP and cTnT expression. GHMT-transduced postnatal TTFs were cultured with the indicated EP antagonists (ant) for 1 week. Quantitative data are shown in d and e; n = 4 biologically independent experiments. See also Supplementary Figure 3. f, g FACS analysis for cTnT expression in GHMT-treated TTFs from WT or Ptger4^−/− mice with or without EP4 antagonist, diclofenac, or PGE2. Quantitative data are shown in g; n = 4 biologically independent experiments. Student’s t-test was performed for g, one-way ANOVA with Dunnett’s post hoc test was performed for b, d, one-way ANOVA with Tukey’s post hoc test was performed for e; all data are presented as mean ±SEM *P < 0.05, **P < 0.01 vs. the relevant control. NS, not significant. Diclo, diclofenac