Fig. 1.
AAVS1-targeted KLF1 transgene is expressed in iPSC-DMs. a Expression of ‘EI’ macrophage related transcription factors (c-MAF and KLF1) in monocyte-derived macrophages (MDMs) (grey bar) and iPSC-derived macrophages (purple bar) by qRT-PCR analyses (n = 5, Mann Whitney test). b Differentiation protocol used to generate macrophages from an iPSC line (iKLF1.2) carrying the AAVS1-targeted KLF1-ERT2 transgene. c Kwik-Diff-stained cytospin preparations of iKLF1.2-derived macrophages (iKLF1.2 MΦ) (scale bar, 20 μm). d Real-time PCR analysis of KLF1 in MDMs , control iPSC-DMs (control Mϕ) and iKLF1.2-DMs (iKLF1.2 MΦ) (n = 4 biologically independent samples) Kruskal–Wallis test with Dunn’s post-test). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001