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. 2019 Feb 20;10(3):175. doi: 10.1038/s41419-019-1382-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a After circTADA2A-E6 or empty vectors were transfected in MDA-MB-231 cells, the localization of endogenous or ectopic circTADA2A-E6 was detected by RNA in situ hybridization (FISH). Red, Cy3-labeled probes specific to circTADA2A-E6; blue, DAPI stain for nuclei. Scale bar, 20 μm. b A schematic drawing showing the putative binding sites of the miRNAs associated with circTADA2A-E6. miR-214 family members (miR-214-3p, miR-761, miR-3619-5p, and miR-4291) share the same binding site as miR-302 family members (miR-302c-3p, miR-520d-3p, and miR-520f-3p) and miR-342 family members (miR-342-3p and miR-1229). c After co-transfecting phRluc-circTADA2A-E6 plasmids with miRNA mimics in 293T cells, Renilla luciferase activity was measured. d FISH analysis on the co-localization of circTADA2A-E6 and miR-203a-3p in MCF-7 cells. Red, Cy3-labeled probes specific to circTADA2A-E6; green, FITC-labeled probes specific to miR-203a-3p; blue, DAPI stain for nuclei. Scale bar, 20 μm. e The circTADA2A-E6/miR-203a-3p and miR-302c-3p/mRNA axes were generated after Cytoscape analysis. f After transfecting MCF-7 cells with the circTADA2A-E6 vector, siRNA #1 or #2, miR-203a-3p mimic, or miR-203a-3p inhibitor, the expression of SOCS3 was analyzed by western blotting (WB)