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. 2019 Feb 20;9:2412. doi: 10.1038/s41598-019-38672-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Peripheral smear of CML patient by using Leishman staining showed large granulocytic cells. (b) The BCR/ABL1:t(9;22) FISH probe (Vysis) in the Interphase cell showing 1 fusion, 1 Orange and 1 Green signals indicating the BCR/ABL: Ph-positive (9q deletion variant) status. (c) Reverse transcriptase-polymerase chain reaction of BCR-ABL transcript variants in 1.5% agarose gel electrophoresis. Lane M: 100 bp ladder, Lane L1, L2, L4 and L5: CML patients samples showing b3a2 variant with 417 bp size, Lane L3 and L6: CML patients samples showing b2a2 variant with 342 bp size. (d) The 1.5% agarose gel showing Lane M: 100 bp ladder, Lane L1-L7: CML patients samples showing b2a2 variant with 342 bp size.