Figure 2.

IFN-γ inhibits RFP expression, a lytic marker of KSHV lytic replication, in a dose-dependent manner. iSLK.219 cells were induced without or with Dox (0.2 μg/ml) in the absence/presence of IFN-γ or TNF-α (final 8 to 5000 units/ml) for 2 days before flow cytometry. Live cells were gated for RFP, whose expression was driven by the polyadenylated nuclear (PAN) RNA promoter. Data are presented as mean ± SEM from three independent experiments. *P < 0.01 by one-way ANOVA Tukey.