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. 2019 Feb 20;9:2320. doi: 10.1038/s41598-019-38568-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Brg1 knockout mice show normal liver development. (A,B) Protein expression of Brg1 in the liver of 2-month-old Control and Brg1 KO mice analysed by Western blot. Representative gels (A) and densitometric analyses (B) are depicted. n = 3. (C) mRNA expression of Brg1 of 2-month-old Control and Brg1 KO mice in the liver analysed by qPCR, n = 3. (D) H&E staining of the liver of 2-month-old Control and Brg1 KO mice. (E) Representative liver morphology of 2-month-old Control and Brg1 KO mice. (F) Immunohistochemistry for Brg1 of the liver of 2-month-old Control and Brg1 KO mice. (G) Liver to body weight ratios of 2-month-old Control and Brg1 KO mice. n = 6. (H) Representative immunohistochemistry images for BrdU of 2-month-old Control and Brg1 KO mice livers. (I) Quantification of cell number (HPF) of 2-month-old Control and Brg1 KO mice, n = 6.