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. 2019 Feb 20;9:2345. doi: 10.1038/s41598-018-38380-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Light chain and heavy chain pair analysis using reduced and non-reduced Fab fragments. (A) The base peak chromatogram of non-reduced Fab mixture with selected MS spectra of three intact Fabs; (B) An example of a light chain and heavy chain pair. High resolution mass spectra of intact Fab and its corresponding light chain and heavy chain were shown above with detected accurate masses. M_mono represents the monoisotopic mass.