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. 2019 Feb 20;9:2345. doi: 10.1038/s41598-018-38380-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The UPLC-TD-HRMS analysis of human serum antibodies. (A) Summed mass spectra of two control samples (top) and the SLE serum sample (bottom). Our results suggested that serum antibody Fabs are difficult to resolve without separation. (B) is the comparison between two control samples using deconvoluted LC-MS mass features from 22,500 Da to 25,000 Da; (C) is the comparison between one control sample and one SLE serum sample using deconvoluted LC-MS mass features from 22,500 Da to 25,000 Da. Some of the high resolution mass spectra of the deconvoluted mass features are also shown above.