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. 2019 Jan 10;116(4):610–620. doi: 10.1016/j.bpj.2019.01.005

Figure 3.

Figure 3

2JNH-HSQC of 15N-labeled N-terminal PrP and full-length PrPC. 1H-15N-HSQC spectra with pulse sequence modified to highlight 2JNH couplings showing the imidazole region of the spectrum for mouse PrP(23–125) (red, correlated crosspeaks connected by dashed lines) and full-length PrP (blue, peaks connected by solid lines). The pattern of connections from Nδ1-Hε1 → Nε2-Hε1 → Nε2-Hδ2 is characteristic of the protonation at Nε2. Crosspeaks from the octarepeat (OR) His residues are labeled “OR.”