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. 2019 Jan 10;116(4):610–620. doi: 10.1016/j.bpj.2019.01.005

Figure 4.

Figure 4

Determination of Kd from 2JNH HSQC chemical shifts versus Cd2+. (A) Nε2-Hδ2 and (B) Nε2-Hε1 2JNH HSQC crosspeaks of N15-labeled PrP (23–125) shift as a function of added cadmium chloride. (C) A plot of 15N chemical shift change versus CdCl2. Data were fitted to the Kd expression derived for fast chemical exchange (Materials and Methods) to determine binding constant for Cd2+ to the OR domain (Table 1). Resulting Kd values derived from the Nε2-Hδ2 and Nε2-Hε1 crosspeaks are ∼1.25 and 1.18 mM, respectively.