Fig. 1.

Generation of Sost ER^T2 Cre mice. a A Sost Bac construct containing the 5’ and 3’ regions of the Sost gene (Sost ER^T2 cre) was made, using RP24-178J4 BAC (157Kb) with the Sost gene. Recombineering was used to replace the ATG region with the CreER^T2 frt-kan-frt cassette, and the frt-kan-frt cassette was then removed with I-arabinose inducible flip recombinase in the SW105 bacteria. The digest with EcoRV shows a 11.6 kb band that is only 0.9 kb after insertion of the CreERt2-frt-kan-frt cassette, using the 5’ probe(purple). After removing the kan cassete the EcoRV band goes from 14.2 kb to 12.7 kb with the 3’ probe (bottom right of 1 a). b Representative images from the four founder lines (#1, #2, #3, #4) that were crossed with Ai9 tdTomato mice to analyze the DNA recombination in osteocytes after tamoxifen injection (300–555 mg·kg⁻¹). Images of the femur were taken at 20x magnification. The upper panels are brightfield images and the lower panels show TdTomato fluorescence. Scale bar represents 100 µm. Line #2 appeared the most promising and osteocyte specific as compared to the other lines