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. 2019 Feb 20;9:2402. doi: 10.1038/s41598-019-38694-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pH (a) and temperature (b) on PLD activity of commercial enzyme. PLD activity was assayed at various pH values using Tris-HCl and citrate-phosphate buffer. PLD activity was assayed after equilibration of assay mixture at a given temperature for 10 minutes. Reaction was started then and allowed for 10 minutes for release of choline.