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. 2019 Feb 20;9:2401. doi: 10.1038/s41598-019-38925-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mutations identified in chemostat-derived E. coli ATCC 25922 polymorphic populations. Genome visualization and comparison of final day unexposed, ITC-exposed and LVX-exposed populations to E. coli reference genome (CP009072) was performed using BRIG. From inner to outer rings: reference genome, unexposed thawed culture at day 20 (CTRD20TC), ITC-exposed thawed culture at day 20 (ITCD20TC) and LVX-exposed thawed culture at day 20 (LVXD20TC). The gaps shown indicate where the sequence data from chemostat populations differ from the reference sequence.