Figure 2.

Core calcium signaling components modulate wing size, shape, and vein differentiation. (A) Schematic representing mechanism of the core IP₃R-mediated Ca²⁺ signaling pathway is shown. ER, endoplasmic reticulum; Gαq, G protein α q subunit; GJ, gap junction; GPCR, G protein-coupled receptor; IP₃, inositol trisphosphate; IP₃R, IP₃ receptor; PLC, phospholipase C, which includes multiple isoforms: PLCγ homolog (sl) and PLCβ homologs (Plc21C and norpA); RTK, receptor tyrosine kinase; SERCA, sarco/endoplasmic reticulum Ca²⁺-ATPase. (B) Schematic of expression pattern of MS1096-Gal4, which is expressed more strongly in the dorsal compartment, is shown. The GAL4/UAS system was used to express RNAi constructs starting during the third instar larval stage. Adult wing phenotypes were used to provide a readout of final phenotype. (C–H) Micrographs of adult fly, orthogonal view of wing, and mounted view of wing for indicated crosses are shown. Scale bars represent 0.5 mm. (I) Total area of adult wings under various perturbations of Ca²⁺ signaling genes driven by MS1096-Gal4. The gray boxes indicate standard deviation. ns, not significant. ^∗p < 0.05; ^∗∗∗p < 0.001 by t-test after Bonferroni correction. The red band provides the average and SD of multiple control crosses. To see this figure in color, go online.