Biolayer interferometry (BLI) spectra for SLU-417, an analog of SLU-258, binding to full-length AcrA, SLU-417 binding to a truncated variant of AcrA lacking the MP domain (AcrA (45–312)), and clorobiocin binding to AcrA (45–312). Experimental data are shown in blue, green, and back lines. The data were fitted into a simple 1:1 binding model, and the fitted lines are shown in red. To see this figure in color, go online.