FIG 1.

Halo assays with C. albicans strains. Halo assays were performed by plating a lawn of opaque a cells on SC-glucose plates and spotting α-pheromone (1 μg) and a control (50% methanol) onto each plate. Pheromone was spotted on the center of each of three circles drawn on the plates, and the 50% methanol control was spotted on the fourth spot. Plates were incubated at room temperature for 2 days for the lawn of cells to grow and then were photographed. Halo activities of opaque cells from mutants were compared to wild-type strains. Cpp1Δ/Δ, GR001, and GR002 are hyperresponsive to pheromone treatment as they generate large halos compared to the wild-type strain, which generates almost imperceptible halos in a standard halo assay test. The size of the halos was measured over the plates.