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. 2019 Feb 20;4(1):e00598-18. doi: 10.1128/mSphere.00598-18

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Copyright © 2019 Rastghalam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Pheromone response assay. Opaque cells were treated with α-pheromone for 3 hours, 6 hours, and 24 hours. This image shows only the results for 24 h of treatment. Cells were observed under a Leica DM6000 microscope. α-pheromone does not induce conjugation tube formation (shmoos) in opaque cells of the Cek1Δ/Δ and GR004 mutants after 3 h and 6 h of treatment, and less than 0.1% of cells showed tube formation after 24 h of treatment. GR003 never induced conjugation tube formation. Arrows designate conjugation structures.