TABLE 1.
Strains used in this study
| Strain | Parent | Mating type |
Description | Genotype | Source or reference |
|---|---|---|---|---|---|
| CAI4 MTLa | CAI-4 | a/a | ura3::imm434/ura3::imm434 | Wild type | Doreen Harcus |
| CP29-1-7L4 | CAI-4 | a/α | ura3/ura3 cpp1::hisG/cpp1::hisG | 26 | |
| Cpp1Δ/Δ | CP29-1-7L4 | a/a | ura3/ura3 cpp1::hisG/cpp1::hisG | cpp1Δ/Δ | This study |
| CK43B-16L | a/α | ura3/ura3 cek1::hisG/cek1::hisG | 14 | ||
| Cek1Δ/Δ | CK43B-16L | a/a | ura3/ura3 cek1::hisG/cek1::hisG | cek1Δ/Δ | This study |
| GR001 | CAI4 MTLa | a/a |
ura3/ura3 cek2::TAA (32nd and 37th amino acids)/cek2::TAA (32nd and 37th amino acids) |
Cek2-stop/stop | This study |
| GR002 | Cpp1Δ/Δ | a/a |
ura3/ura3 cpp1/cpp1; cek2::TAA (32nd and 37th amino acids)/cek2::TAA (32nd and 37th amino acids) |
Cpp1Δ/Δ Cek2-stop/stop | This study |
| GR003 | Cek1Δ/Δ | a/a |
ura3/ura3 cek1/cek1; cek2::TAA (32nd and 37th amino acids)/cek2::TAA (32nd and 37th amino acids) |
Cek1Δ/Δ Cek2-stop/stop | This study |
| GR004 | Cpp1Δ/Δ | a/a |
ura3/ura3 cpp1/cpp1; cek1::TAA
(19th and 25th amino acids)/cek1::TAA (19th and 25th amino acids) |
Cpp1Δ/Δ Cek1-stop/stop | This study |
| Cpp1Δ/Δ (Cek1-GFP) | Cpp1Δ/Δ | a/a | Cpp1Δ/Δ (Cek1-GFP) | This study | |
| CAI4, Cek1-GFP | CAI-4 | a/a | Cek1-GFP | This study | |
| Cpp1Δ/Δ (Cek2-GFP) | Cpp1Δ/Δ | a/a | Cpp1Δ/Δ (Cek2-GFP) | This study | |
| CAI4 (Cek2-GFP) | CAI-4 | a/a | Cek2-GFP | This study | |
| 3315 | A505 | α/α | trp1/trp1; lys2/lys2 | P. T. Magee | |
| SN148 | RM1000#2 | a/α |
arg4/arg4; leu2/leu2; his1/his1; ura3 imm434/ura3 imm434; iro1 imm434/iro1 imm434 |
Suzanne Noble/Sandy Johnson |