TABLE 2.
Percentage of projection formation for different strainsa
| Strain | Duration of pheromone treatment (h) | No. of cells showing morphological projection | % of projection formation |
|---|---|---|---|
| CAI4 | 3 | 57 | 50 |
| 6 | 81 | 85 | |
| 24 | 100 | 100 | |
| Cpp1Δ/Δ mutant | 3 | 91 | 94 |
| 6 | 98.5 | 100 | |
| 24 | 100 | 100 | |
| Cek1Δ/Δ mutant | 3 | 2 | 1.5 |
| 6 | 2.1 | 4 | |
| 24 | 7.5 | 10 | |
| GR001 | 3 | 21 | 28 |
| 6 | 56.5 | 58 | |
| 24 | 90.5 | 92 | |
| GR002 | 3 | 95.5 | 98 |
| 6 | 99 | 100 | |
| 24 | 100 | 100 | |
| GR003 | 3 | 0.00 | 0.00 |
| 6 | 0.00 | 0.00 | |
| 24 | 0.00 | 0.00 | |
| GR004 | 3 | 2.4 | 5 |
| 6 | 4 | 8 | |
| 24 | 9.5 | 12 |
a
The percentage of projection formation was computed by counting the number of cells showing morphological projections after treating the cells with pheromone for 3, 6, and 24 h divided by the total number of cells counted (∼150 cells for each strain) and multiplying by 100. All cells were of the opaque type.