Figure 2.

Region-specific increase in E2 levels following 4 weeks of letrozole (20 μg/d, p.o.). Brain regions analyzed were as follows: (A) hippocampus, (B) hypothalamus, and (C) frontal cortex. Letrozole (males: n = 4; females: n = 3) was associated with higher E2 levels in the hippocampus relative to the control group (males: n = 4; females: n = 4). *p < 0.05. Remaining regions were unaffected by drug treatment. There was no effect of sex on E2 levels of any region. Mean values obtained in each group are presented in pg/g. Error bars indicate SEM. Dashed horizontal lines indicate the average background E2 levels obtained by EIA for assay blanks.