Figure 5.

Cortical synaptic zinc signaling improves sound frequency discrimination. a, Schematic illustrating the placement of bilateral chronic cannulas over the auditory cortex (ACx) for local ZX1 infusion. Cartoon of brain adapted from (Paxinos and Fraklin, 2001). b, Examples of ASR waveforms after ZX1 (red) or vehicle (black) infusions. c, Summary graph showing ASR amplitudes after ZX1 (red) or vehicle (black) infusion in WT (normalized to 16 kHz F2: for vehicle or ZX1 vs 16 kHz, p ≤ 0.005, n = 5 mice, one-sample t tests). d, Examples of ASR waveforms after different prepulse stimuli before (control, black) and after (red) ZX1. e, Summary graph showing prepulse inhibition for different prepulse stimuli before (control, black) and after (red, n = 5 mice) ZX1 infusion. f, Same as in e but in ZnT3 KO (p ≤ 0.005, n = 4 mice, one-sample t tests). For all panels, asterisks indicate significant differences after accounting for multiple comparisons using the Holm-Bonferroni's correction; error bars indicate SEM. Detailed values and staticistcal test are listed in the Materials and Methods.