Table 2.
Rating of behavioral signs of withdrawal
| CTRL |
EtOH-WDL 12 h |
EtOH-WDL 48 h |
EtOH-WDL 14 d |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Median | IQR | Median | IQR | p | Median | IQR | p | Median | IQR | p | |
| Global rating | |||||||||||
| Vehicle | 0.0 | 0.0–0.0 | 3.0 | 3.0–4.5 | *** | 2.0 | 1.0–2.0 | * | 0.0 | 0.0–0.5 | n.s. |
| l-DOPA | 0.0 | 0.0–0.0 | 2.0 | 1.0–2.0 | ∧∧ | 1.0 | 0.5–1.0 | n. s. | 0.0 | 0.0–0.5 | n.s. |
| Individual signs | |||||||||||
| Body tremors | |||||||||||
| Vehicle | 0.0 | 0.0–0.0 | 0.5 | 0.0–1.0 | * | 0.0 | 0.0–0.5 | n. s. | 0.0 | 0.0–0.0 | n.s. |
| l-DOPA | 0.0 | 0.0–0.0 | 0.0 | 0.0–1.0 | n. s. | 0.0 | 0.0–0.0 | n. s. | 0.0 | 0.0–0.0 | n.s. |
| Tail stiffness | |||||||||||
| Vehicle | 0.0 | 0.0–0.0 | 1.0 | 1.0–2.0 | ** | 1.0 | 0.5–2.0 | * * | 0.0 | 0.0–0.0 | n.s. |
| l-DOPA | 0.0 | 0.0–0.0 | 0.5 | 0.0–1.0 | n. s. | 0.0 | 0.0–0.0 | n. s. | 0.0 | 0.0–0.0 | n.s. |
| Irritability to touch | |||||||||||
| Vehicle | 0.0 | 0.0–0.0 | 2.0 | 1.0–2.0 | *** | 0.5 | 0.0–0.5 | n. s. | 0.0 | 0.0–0.5 | n.s. |
| l-DOPA | 0.0 | 0.0–0.0 | 1.0 | 0.5–1.0 | ∧ | 1.0 | 0.0–1.0 | n. s. | 0.0 | 0.0–0.5 | n.s. |
Each individual sign of withdrawal was rated on a 0–2 point scale, with 0 representing absence of sign, 1 representing moderate severity, and 2 representing extreme severity. Individual withdrawal sign ratings were then combined to produce a global score. Data are indicated as median and interquartile range (IQR) of n = 8 rats.
***p < 0.001,
**p < 0.01,
*p < 0.05 Dunn's post hoc test versus CTRL;
∧∧p < 0.01,
∧p < 0.05 two-tailed Mann–Whitney U test versus vehicle.