TABLE 1.

Comparison of different biodistribution studies of T cells in mice. Methods of T cell isolation, expansion, and labeling, as well as tissue biodistribution at 20-24 hours are compared.

Method	Wallace et al. (1993)		Melder et al. (2002)		Present Study
Labeling	I-125 PKH95–labeled T cells		In-111 oxine–labeled T cells		Cr-51–labeled T cells
Preparation of T cells	Splenocytes exposed to 6000 IU/ml IL-2	TILs extracted from MC38 s.c. tumors	Lymphocytes extracted from spleen	Mice sensitized with splenocytes of MCaIV tumor-bearing C3H mice and lymphocytes extracted from spleen	Splenocytes expanded using anti-CD3, anti-CD28, and IL-2	TILs extracted from B16-BL6 s.c. tumors
Tissues listed in descending order of concentrations at 20–24 h	Spleen	Lung	Spleen	Lung	Spleen	Lung
	Lung	Spleen	Liver	Spleen	Liver	Spleen
	Liver	Liver	Lung	Liver	Lung	Liver
	Skin	Skin	Tumor	Tumor	Kidney	Kidney
	Muscle	Muscle	Heart	Heart	TDLN	Tumor