Figure 4.

Fecal transplantation alters protein expression of Slc26A6 transporter involved in secretion of oxalate. (A) Western blot for the Slc26A6 transporter in kidney, ileum, cecum and colon tissues harvested from germ‐free age match controls and fecal transplanted mice at 4‐weeks after transplanted. Average Slc26A6 receptor protein levels, indicating that in fecal transplanted animals, Slc26A6 expression in the kidney decreased by 40.25% (P = 0.0137). There was no significant difference in expression observed in the ileum. Cecum Slc26A6 increased by 90% as compared to control (P = 0.0005) and colon Slc26A6 decreased by 38.50% (P = 0.0051) when compared to controls. Protein band intensities were first normalized by the GAPDH loading control and then by their respective controls and expressed as mean ± SEM; n = 6 age match controls and 8 fecal‐transplanted mice (1 mouse died prior to tissues collection); Student's t‐test compared to controls: *P < 0.05, **P < 0.01 and ***P < 0.001.