Figure 2.

Hydrostatic Pressure Increases during Mitosis, Peaking Right after Anaphase Onset

(A–J) (A) Mean rounding force (magenta line) and mean hydrostatic pressure (black balls) measured by AFM using the parallel plate assay (see Figure S2 for more details; error bars represent standard error of the mean, n = 13) throughout mitosis for wild-type neuroblasts. The boxed measurements are shown enlarged in (B). The gray vertical line refers to anaphase onset (0 min). The orange vertical line highlights the onset of the hydrostatic pressure drop. Graphs showing the mean cortical stiffness and mean hydrostatic pressure throughout mitosis for (C) the entire neuroblast (global average) and the (D) apical, (E) furrow, and (F) basal regions. Graphs showing the mean cortical stiffness and mean Myosin intensity throughout mitosis for (G) the entire neuroblast (global average) and the (H) apical, (I) furrow, and (J) basal regions.