Fig. 4.
The role of PP2A/MAPKs/HIF-1α pathway in the neuroprotective effect of FGF21 against tau pathology and oxidative stress damage. The phosphorylation and total levels of PP2A, ERK1/2, p38 and JNK, and the expression levels of HIF-1α in rats’ hippocampus (A) and in SH-SY5Y cells (B) were measured by western blotting. The effects of inhibitor PD98059 on the phosphorylated-ERK1/2, total ERK1/2, Thr181-phosphorylated tau, Thr205-phosphorylated tau, total tau (C) and ROS production (D) in different groups with or without Aβ25–35 /FGF21 were shown. All experiments were repeated at least three times. #P < 0.05, ##P < 0.01 and ###P < 0.001 compared with the control group; * P < 0.05, * * P < 0.01 and * ** P < 0.001 compared with the Aβ25–35 group.