Figure 4.

ChIP-Seq Analysis Reveals Ring1b Targets in the Wnt Pathway

(A and B) GO enrichment analysis using PANTHER pathway identified Wnt/β-catenin signaling as the top pathway in Ring1b (A) and H3K4me3 (B) ChIP-seq datasets.

(C) Peak calling showed the enrichment of Wnt target genes co-marked by Ring1b and H3K4me3 but no enrichment with H3K27me3.

(D) Validation of downregulated Wnt target genes by Ring1b using real-time PCR. N ≥ 3 mice per group. p < 0.05 by Student’s t test. Data presented as means ± SEMs.

(E) Genomic view of Zic1/2 co-occupying the same loci as Ring1b and H3K27me3 rather than H3K4me3.

(F) Zic1/2 were upregulated following deletion of Ring1a/b identified by real-time PCR.

N ≥ 3 mice per group. ^∗∗∗p < 0.001 by Student’s t test. Data presented as means ± SEMs.