Figure 6.

Maintenance of Stem Cell Stability by PRC1

(A) Schematic illustration demonstrated EdU retention assay to detect slow-cycling stem cells.

(B) EdU⁺ cells were distinctly localized between the labial and lingual aspects of the cervical loop, but they disappeared by 96 hr post-tamoxifen induction of Ring1 deletion.

(C) Tunel assays revealed apoptotic cells in the stem cell zone in Ring1⁻ cells, whereas no obvious apoptosis was detected in control incisors.

(D) Reduced cell proliferation was detected in Wls^flox/flox;Axin2^CreERT2 marked by BrdU-labeled cells.

(E) Annexin V⁺ apoptotic cells were visible in the stem cell zone 2 days post-tamoxifen on Ring1a^−/−;Ring1b^flox/flox:Axin2^CreERT2 mice compared to controls.

N ≥ 3 mice per group.