Figure 1.

Representation of Limb Perfusion Routes and Images of the Dosed Limbs for Each Route of Delivery

(A) Graphical representation of venous limb perfusion (left) and intra-arterial push and dwell (right). (B–D) VLP dosed limb (B) at the start of delivery, (C) 5 min into the vector infusion, and (D) after all vector had been infused but tourniquet had not been released. (E) Quadriceps dosing sight; note that intra-muscular (IM) injection sights are denoted using a black marking pen. The animal’s body is to the left of the image and the knee is to the right. (F) Gastrocnemius dosing sight; note that IM injection sights are denoted using a black marking pen. The animal’s knee is to the left of the image, and the foot is to the right. (G) Intra-arterial push and dwell (IAPD)-dosed limb at the end of infusion before balloon catheters deflated (the image shows the inner aspect of the dosed limb).