Figure 2.

Serum AAT c-myc Expression, Muscle Immunohistochemistry for AAT, and RT-PCR

(A) Western blot gel image for all time points (please note that several AAV8 day-60 samples were missing). (B) Semi-quantitation of western blot (WB) data represented as mean ± SEM. (C) Immunohistochemistry for AAT at day-60 representative sections for each dosing route for both AAV1 and AAV8. Muscle samples were collected for immunohistochemistry (IHC) from the level of the IM injections in both the quadriceps and gastrocnemius. (D) RT-PCR data from day-60 muscle and liver tissue. The gastrocnemius muscle samples were used for all groups. IM muscle samples were collected from the injection site. IM, intramuscular; IAPD, intra-arterial push and dwell; VLP, venous limb perfusion.