Fig. 8.

Fibrosis develops with the repeated-dosing regimen of cisplatin (0 or 7 mg/kg once a week for 4 wk) in 8- and 40-wk-old male FVB mice. A: protein levels of transforming growth factor-β (TGFβ) and fibronectin in kidney cortex homogenate were determined by Western blotting, and TGFβ protein levels were quantified. B: total collagen levels were determined by Sirius red/fast green staining and quantified as percent Sirius red-positive pixels. C: presence of myofibroblasts was determined by α-smooth muscle actin (α-SMA) immunohistochemistry and quantified as percent α-SMA-positive pixels. Values are means ± SE; n = 3–4. Statistical significance was determined by 2-way ANOVA followed by Tukey’s post test.